C2493
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 | Immunoblotting Whole cell lysates were separated on SDS-PAGE and blotted with Anti-CENP-A (N-terminal) (Cat. No. C2493). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:4,000 Lanes 1: HEK-293T cells transfected with human CENP-A 2: Recombinant human CENP-A protein 3: Untransfected HEK-293T cells 4: HEK-293T cells transfected with human CENP-A + Inhibition with CENP-A immunizing peptide (human 4-17) (20 μg/mL) |
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C2493
Sigma
Anti-CENP-A (N-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-CenH3, Anti-Centomeric protein A |
Description
| Immunogen | synthetic peptide corresponding to amino acids 4-17 located at the N-terminus of human CENP-A. |
| Physical form | Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 17 kDa |
| species reactivity | human, bovine (predicted) |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using HEK-293T cells expressing human CENP-A |
| Gene Information | human ... CENPA(1058) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
